Genetic Engineering Tutorial

Chapter 14 Genetic design Choose the best answer for each question. 1. Using this key, put the phrases in the correct order to ricochet a plasmid carrying the recombinant desoxyribonucleic acid. Key 1) use restriction enzymes 2) Use desoxyribonucleic acid ligase 3) Remove plasmid from farm bacterium 4) Introduce plasmid into new host bacterium. A. 1, 2, 3, 4C. 3, 1, 2, 4 B. 4, 3, 2,1 D. 2, 3, 1, 4 2. Which is not a dead ringer? A. a colony of identical bacterial kiosks B. identical quintuplets C. a forest of identical trees D. eggs produced by oo agentsis E. copies of a gene through PCR 3.Restriction enzymes found in bacterial cells ar ordinarily used A. during DNA replication B. to degrade the bacterial cells DNA C. to degrade viral DNA that enters the cell D. to attach pieces of DNA together 4. Recombinant DNA technology is used A. for gene therapy B. to clone a gene C. to make a particular protein D. to clone a specific piece of DNA E. all told of these are correct 5. In order for bacterial cells to express human genes, A. the recombinant DNA must not forbear introns. B. reverse transcriptase is sometimes used to make complementary DNA from an mRNA molecule.C. bacterial regulative genes must be included. D. All of these are correct. 6. The polymerase chain reaction A. utilizes RNA polymerase B. takes place in grand bioreactors C. utilizes temperature insensitive enzyme D. makes lots of nonidentical copies of DNA E. All of these are correct 7. DNA fingerprinting can be used for which of these? A. identifying human remains B. identifying infectious diseases C. finding evolutionary links between organisms D. solving crimes E. All of these are correct 8. DNA amplified by PCR and then used for fingerprinting could come from A. ny diploid or haploid cell B. only white blood cells that have been karyotyped C. only skin cells after they are dead D. only purified animal cells E. both B and D are correct 9. Which of these pairs is incorrectly matched? A. D NA ligase DNA fingerprint B. Restriction enzymes Cloning C. DNA fragments DNA fingerprinting D. DNA polymerase PCR 10. Which of these is an incorrect recital? A. bacteria secrete the biotechnology product into the medium B. plants are being engineered to have human proteins in their seeds. C. nimals are engineered to have a human protein in their milk. D. animals can be cloned, but plants and bacteria cannot. 11. Which of these is not needed in order to clone an animal? A. sperm from a donor animal B. magnetic core from an adult animal cell C. enucleated egg from a donor animal D. host female to commence the embryo E. All of these are needed 12. Because the human genome Project, we know or will know the A. date of the base pairs of our DNA B. sequence of genes along the human chromosomes C. mutations that lead to genetic disorders D.All of these are correct 13. The restriction enzyme called EcoRI has cut double stranded DNA in the following manner. The piece of foreign DNA t o be inserted has what bases from the left and from the right? 14. Which of these is a true statement? A. Plasmids can serve as vectors B. Plasmids are analog DNA found in viruses C. Plasmids can replicate in the host cell D. Both A and C are correct 15. Which of these is a benefit of having insulin produced by biotechnology? A. It is just as impressive B. It can be mass produced C. It is less expensive D. All of the above